Bioremediation of crude oil contaminated soils using. It is a type of waste management technique which involves the use of organisms to remove or utilize the pollutants from a polluted area. Epas citizens guide to bioremediation epa 2012a to be a more fundamental and concise reference. Characterization of furnace oil bioremediation potential. The abundance of different hydrocarbonoclastic bacteria was assessed in the creosote contaminated soil samples before the inoculation and after the bioremediation of the soil samples.
The primary factors restricting the biodegradation efficiency of. Potential hydrocarbonoclastic bacteria biotech articles. Characterization of furnace oil bioremediation potential of hydrocarbonoclastic bacteria isolated from petroleum contaminated sites of the sundarbans, bangladesh. This research is aimed at isolating hydrocarbonoclastic bacteria from petroleum refinery effluent and also assessing their bioremediating potentials in hydrocarbon cleanup programmes. Both of them are called hydrocarbonoclastic, which. Bioremediation of crude oil contaminated sediment using. The key players in bioremediation are bacteriamicroscopic organisms that live virtually everywhere. Petroleum effluent is discharged into river rido and this poses a threat to the communities that utilize the water downstream of river rido, the ultimate recipient of the effluent. Efficacy of intervention strategies for bioremediation of. Pdf the present study was undertaken to evaluate the biodegradation potential of the indigenous bacterial population of cudda10 re fishing harbour. Interest in the microbial biodegradation of pollutants has intensified in recent years, and recent major methodological b. Bioremediation of crude oil contaminated soils using surfactants and hydrocarbonoclastic bacteria. Hydrocarbons are principle constituents of petroleum, related byproducts like coal tar, diesel, petrol, gasoline. Most hydrocarbonoclastic bacteria in the total environment are.
Bioremediation process types of bioremediation examples. Pdf bioremediation potential of hydrocarbonoclastic bacteria in. The dynamics of the hydrocarbonoclastic bacterial communities in the heaps was monitored. Bioremediation of soils saturated with spilled crude oil.
Applications of bioremediation and phytoremediation article pdf available in science progress 96pt 4. Oilbioremediation potential of two hydrocarbonoclastic, diazotrophic marinobacter species in hypersaline areas along the arabian gulf coasts. This singlesource reference encompasses all categories of pollutants and their applications in a convenient, comprehensive package. Microbial biodegradation is the use of bioremediation and biotransformation methods to harness the naturally occurring ability of microbial xenobiotic metabolism to degrade, transform or accumulate environmental pollutants, including hydrocarbons, polychlorinated biphenyls, polyaromatic hydrocarbons, heterocyclic compounds, pharmaceutical substances, radionuclides and metals. Marine hydrocarbonoclastic bacteria and their function in bioremediation of marine oil spills. Pdf eightytwo out of the 100 hydrocarbonoclastic bacterial species that have. Bioremediation as a tool for resiliency in oil spills.
Hydrophobic nitrogen and phosphorus fertilizers are the potential solution for the treatment of oil spills. Bioremediation of crude oil contaminated sediment using slow release fertilizer. In the course of our work on bioremediation of desert and aquatic ecosystems that had been heavily polluted with oil during the greatest manmade oil spill at the end of the 1991 gulfwar, we used to isolate hydrocarbonoclastic bacteria that according to their 16s rrnagene sequences were coliforms. Bioremediation of soils saturated with spilled crude oil scientific. Autochthonous bioaugmentation with environmental samples. Hindu college, nagercoil, tamil nadu, india key words. These bacteria can only metabolize a few organic acids acetate. Anaerobic bacteria are not as frequently used as aerobic bacteria.
Bioremediation bioremediation is the use of microorganisms to destroy or immobilize waste materials microorganisms include. Bioremediation as a tool for resiliency in oil spills abstract with an increase in boating, offshore drilling, and transportation of oil, coastal communities are in need of a plan to clean up waterways in the event of an offshore oil spill. Most hydrocarbonoclastic bacteria in the total environment are diazotrophic, which highlights their value in the bioremediation of hydrocarbon contaminants. Petroleum microbiology is a branch of microbiology that deals with the study of microorganisms that can metabolize or alter crude or refined petroleum products. Enimie endurance oaikhena 1, dominic bawa makaije 1, samuel denwe 1, muhammad muktar namadi 2, omolola elizabeth fatanmi 1.
The mechanism by which hydrocarbonoclastic bacteria degrade. We concluded that most hydrocarbonoclastic bacteria are diazotrophic, which allows for their wide distribution in the total environment. In the present study, we isolated and characterized three superior hydrocarbonoclastic bacteria from river water, soil sediments, and submerged barks of plant samples collected from the sundarbans oil spillage area and assessed their oil pollutant bioremediation ability in vitro. Bioremediation techniquesclassification based on site of application. For conducting the bioremediation capability of the isolates and other oil. Hydrocarbonoclastic bacteria pdf august 15, 2019 specialized hydrocarbonoclastic bacteria shcb, which use limited carbon sources with a preference for petroleum hydrocarbons, were found. Hydrocarbonoclastic means degrading hydrocarbons or simply eating or breaking the hydrocarbon molecules. Petroleum, crude oil or oil and hydrocarbons are sometimes used synonymously. Each value was the mean of five parallel replicates. The hydrocarbonoclastic potential of strain mk2 was evaluated. Overall, this study demonstrates that the potential for pah degradation exists for the bioremediation of hamada cotbscontaminated environments in libya. The bacteria are already there they are just stimulated to encourage growth and activity. The first type of bioremediation is biostimulation.
Mixed culture consortium of all five bacteria isolates had the highest bioremediation potential of 68% as against the single inoculations of pseudomonas aeruginosa, serratia marcescens, klebsiella pneumoniae, proteus vulgaris and streptomyces spp. Consideration should be given to seeding oil spills with hydrocarbonoclastic bacteria. Bioremediation, contaminants, monitoring and saturation received 16 march 2011. The bioremediation potential of hydrocarbonoclastic.
Bioremediation can be defined as the ability of certain biomolecules or types of biomass to bind and concentrate selected ions or other molecules present in aqueous solutions. In particular, in order to find out more about these microorganisms metabolism, biologists have analyzed two different bacteria. In situ bioremediation isb of groundwater involves the encouragement of indigenous bacterial populations to metabolize target contaminants through the addition of various amendments biostimulation to the subsurface environment. Manual removal of oil is a critical first step and when paired with bioremediation. The environmental samples studied were oily seawater and soil samples, that had been bioaugmented with natural materials rich in hydrocarbonoclastic bacteria. Identification of electrode respiring, hydrocarbonoclastic. The top three hydrocarbonoclastic bacterial isolates were selected as potential and. Bioremediation techniquesclassification based on site of. Bioremediation is a biotechnical process, which abates or cleans up contamination. Microorganisms are ideally suited to the task of contaminant destruction because they possess enzymes that allow them to use environmental contaminants as food and because they are so small that they are able to contact contaminants easily. A total of five hydrocarbonoclastic bacteria were isolated from the petroleum refinery effluent. The present study involves the characterization and assessment of biosurfactant producing indigenous hydrocarbonoclastic bacteria and their potential application in bioremediation processes. These hydrocarbonoclastic bacteria are of great importance since they belong to the key players in oil removal from contaminated marine sites. Microbial biodegradation is the use of bioremediation and biotransformation methods to harness the naturally occurring ability of microbial xenobiotic metabolism to degrade, transform or accumulate environmental pollutants, including hydrocarbons e.
Thayib 1978 showed the distribution of hydrocarbonoclastic bacteria in jakarta bay, whereas darmayati 2003, 2004 exhibited in makassar strait and java sea. This represents the first report on the isolation of hydrocarbonoclastic bacteria from libyan cotbs and cotbscontaminated soil. It showed that oil degrading bacteria were present in the marine environment. The marine hydrocarbonoclastic bacterium alcanivorax borkumensis is able to degrade mixtures of n alkanes as they occur in marine oil spills.
The concentration of these bacteria increases significantly in areas of oil spill. Coliform bacteria for bioremediation of waste hydrocarbons. There are several remedies where contaminated water or solid is purified by chemical treatment, incineration, and burial in a. Efficacy of intervention strategies for bioremediation of crude oil in marine systems and effects on indigenous hydrocarbonoclastic bacteria.
Hydrocarbonoclastic bacteria pdf specialized hydrocarbonoclastic bacteria shcb, which use limited carbon sources with a preference for petroleum hydrocarbons, were found. Most hydrocarbonoclastic bacteria in the total environment. Here and in other marine environments polluted by crude oil, marine hydrocarbonoclastic bacteria were detected as key players in bioremediation 1. Microbial ore leaching is the process in which microorganisms are used to extract metals from ores. The dominant group of such bacteria are the hydrocarbonoclastic bacteria hcb. Adegoke 1 1 department of microbiology, university of uyo, uyo, akwa ibom state, nigeria. Pdf most hydrocarbonoclastic bacteria in the total environment. Specialized hydrocarbonoclastic bacteria shcb, which use limited carbon sources with a preference for petroleum hydrocarbons, were found. Bioremediation using microorganisms shows great potential for future development due to its environmental compatibility and possible costeffectiveness.
As one of ex situ bioremediation techniques, windrows rely on periodic turning of piled polluted soil to enhance bioremediation by increasing degradation activities of indigenous andor transient hydrocarbonoclastic bacteria present in polluted soil. Microbial biodegradation and bioremediation 1st edition. Bacterial isolates consisted of bacteria hydrocarbonoclastic and biosurfactant producing bacteria. Vidali encyclopedia of life support systems eolss recent years has led to international efforts to remedy many of these sites, either as a response to the risk of adverse health or environmental effects caused by contamination or to enable the site to be redeveloped for use. This molecular approach revealed much more diverse bacterial taxa than the culture. Three bacterial strains were isolated from various crude oil contaminated environments and characterized using standard identification techniques.
The isolates were inoculated separately into mineral salt medium supplemented with 1% crude oil to determine their. Bioremediation is the use of living organisms, primarily microorganisms, to degrade environmental contaminants. Bioaugmentation failed to enhance oil bioremediation in. The bioremediation potential of hydrocarbonoclastic bacteria isolated from a mangrove contaminated by petroleum hydrocarbons on the cilacap coast, indonesia. Similarly, some obligate hydrocarbonoclastic bacteria ohcb, including.
Petroleum microbiology is a branch of microbiology that deals with the study of microorganisms that can metabolize or. Bioremediation techniques, category definitions, and mode of action in marine and freshwater environments originally compiled to update and revise us epanrt bioremediation fact sheet and rrt iv bioremediation spill response guidance, types of bioremediation section and bioremediation response plan appendix g, in coordination. Growth rates increased with increasing alkane chain length up to a. Eightytwo out of the 100 hydrocarbonoclastic bacterial species that have. Characterization of furnace oil bioremediation potential of. Hydrocarbondegrading bacteria often produce bioemulsifiers. Bioremediation of petroleum in the sea is limited by n and p availability. Introduction to in situ bioremediation of groundwater. Pdf applications of bioremediation and phytoremediation.
Petroleum hydrocarbon spills in the environment and. Narjes dashti, 1 nedaa ali, 1 mohamed eliyas, 1 majida khanafer, 1 naser a. Physiology, biochemistry, genomics conference paper pdf available october 2014 with 400 reads. Changes in the microbial community during bioremediation of.
Bacterial storage lipids are being considered as viable alternative feedstocks for industrial and biotechnological applications, compared to conventional ones. However, investigations of growth behavior and physiology of these bacteria when cultivated with n alkanes of different chain lengths c6 to c30 as the substrates are still lacking. A desert soil sample was saturated with crude oil 17. Oil consumption was measured by gasliquid chromatography. Changes in the numbers of cfus of hydrocarbonoclastic bacteria in the four soil samples during benchscale oilbioremediation. Bioremediation potentials of hydrocarbonoclastic bacteria. Schematic diagram explaining the different microbial hydrocarbon degradation strategy for bioremediation. There is an increasing interest in anaerobic bacteria used for bioremediation of polychlorinated biphenyls pcbs in river sediments, dechlorination of the solvent trichloroethylene tce, and chloroform. Pdf bioremediation techniquesclassification based on.
Hydrocarbonoclastic bacteria in the bioremediation batches were counted using a mineral medium with oil vapor as a sole carbon source and characterized by. Microbial biodegradation and bioremediation brings together experts in relevant fields to describe the successful application of microbes and their derivatives for bioremediation of potentially toxic and relatively novel compounds. Characterization and assessment of biosurfactant producing. Oilcontaminated seawater and desert soil batches were bioaugmented with suspensions of pea pisum sativum rhizosphere and soil with long history of oil pollution. Therefore, these bacteria are useful for the costeffective, environmentally friendly bioremediation of hydrocarbon contaminants.
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